fluorophore-conjugated antibodies.
Synaptophysin (red) and microtubule-associated protein 2 (MAP2; green) in mouse cortical neurons were fluorescently labeled with specific primary antibodies and Thermo Scientific DyLight 649-Conjugated Goat Anti-Rabbit or DyLight 488-Conjugated Goat Anti-Mouse Secondary Antibodies, respectively. Nuclei in both panels were labeled with Hoechst dye.
our body, 20% is absorbed by stomach through blood vessels, and 80% goes to the small intestine. About 10% of those that were absorbed are discharged with sweat, and urine. The rest is detoxified by the liver and metabolized. The communication between neurons that are affected by alcohol gets slower. Therefore, the ability of processing emotion, anger, speech, judgment and memory declines.
Development background
2. History of development
1960s
Discovery of colony-stimulating factors (CSF)
1980s
GM-CSF, M-CSF, G-CSF and multi-CSF
The human G-CSF was cloned
Usage for the treatment at first
1990s
first approval of Filgrastim by the US Food and Drug Administration
Launched Lenograstim
2000s
Launched PEG-filgrastim
I.
1. 문제 제시
35세의 젊은 여자가 2개월 전부터 팔 다리 부종이 나타나고, 전부터 있던 호흡곤란이 악화되어 내원하였다. 1달 전부터 손, 발 피부에 검은 자반이 생겼다고 한다.
2. 단서 인식
- 35세 젊은 여자
- 2달 전부터 팔 다리 부종
- 전부터 있던 호흡곤란 악화
- 1달 전부터 손 발 피부에
Stem cell calssification
1. Based upon location
Embryonic stem cells
Somatic/ adult stem cells
2. Based upon function
Totipotent
Pluripotent
Multipotent
Unipotent
Reject Response
Traditional Immuno-repressents
Constant administration
Little engraftment after 3~4 weeks
Host immune system impaired
Vulnerable to Infection
↓Need brief immuno-re
● NGF, ML-9, ERK, p38MAPK, NF-68 – Sigma ( USA )
● PI3-K – Calbiochem ( Germany )
● LY294002 – TOCRIS ( USA )
● Anti-GSK3β – New England Biolabs ( USA )
● Anti-HRP, anti-p38 – Santa Cruz Biotechnology ( USA )
▼ Phosphorylation of Akt by NGF in PC12 cells
▼ Roles of Akt in PC12 defferentiation in response to NGF
▼ Positive role
3. 암화에 의한 세포주 확립
동물세포를 이용한 연구 발전에 기여
4. Sato등에 의해 무혈청 배양법 확립
1960. 무혈청 배양 시도
1963-5(Ham) 혈청 공급 없이
CHO cell cloning
1970(Sato) 혈청 대신 호르몬
첨가 cell line 무혈청 배양
성공
Human embryonal kidney, SV40 and large T antigen transformed
A. Limitations
So far we have seen a new approach of using stem cells for targeting cancer site and a new concept of
treatment. However it might seem fascinating, it is important to note that significant impediments remain
before the true potential of this novel therapy can be fully realized. Here in this section we are going to
mainly focus on the limitations of neural stem cells esp
의학 약어 사전을 만들면서
외우기 힘들고 낯설은 의학 약어들, 부분에 따라 여러가지로 쓰이는 약어들 때문에 짜증이 나서 하나 둘씩 워드로 치기 시작한 것이 벌써 이만큼이나 되었다. 이중에는 내가 공부하면서 하나씩 하나씩 친것들이 대부분이고, 간혹 가다 눈에 띄는 약어 모음 부록이 있으면